Purification and Properties of Reduced Diphosphopyridine Nucleotide Kinase from Yeast Mitochondria
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منابع مشابه
Purification and properties of reduced diphosphopyridine nucleotide oxidase from Azotobacter.
Few studies on purified bacterial reduced diphosphopyridine nucleotide (DPNH) or cytochrome oxidases have been reported, although cytochrome reduction by DPNH’ in crude bacterial extracts has repeatedly been described (l-6). Dolin (7, 8) purified a flavoprotein DPNH oxidase and peroxidase from Streptococcus jaecalis, and Lenhoff and Kaplan (9) and Lenhoff et al. (10) described the characteristi...
متن کاملPurification and properties of diphosphopyridine nucleotide diaphorase of human erythrocytes.
Diaphorase Assay-Six-hundredths pmole of 2,6-dichlorophenolindophenol, 50 pmoles of Tris-HCl, pH 7.55, 1 pmole of EDTA, enzyme, and 0.44 pmole of DPNH in a volume of 3 ml were placed in a Beckman spectrophotometer equipped with a water jacket through which water at 25” was circulated. A control cuvette without enzyme was included, and absorbancy at 600 rnp was determined for 10 minutes. Under t...
متن کاملThe reduced diphosphopyridine nucleotide oxidase of Streptococcus faecalis: purification and properties.
Extracts of anaerobically grown cells of Streptococcus fuecalis, strain lOC1, contain a reduced diphosphopyridine nucleotide oxidase which catalyzes the reduction of oxygen to hydrogen peroxide (1, 2). Although the enzyme has not been purified, experiments (3) carried out with extracts of the organism grown in riboflavin-deficient media, as well as experiments in which acid-salt resolution of t...
متن کاملEnzymatic conversion of a reduced diphosphopyridine nucleotide derivative to reduced diphosphopyridine nucleotide.
DPNH-X was prepared as described previously (1) ; the barium salt was converted to the sodium salt before use, and solutions were stored at 20”. Concentrations of DPNH-X were calculated with an extinction coefficient of 13.5 X lo6 sq. cm. mole-’ at 290 rnM (1). Sodium salts of ADP, ATP, ITP, UTP, and crystalline disodium ATP were obtained from the Pabst Laboratories, Milwaukee, Wisconsin. Myoki...
متن کاملMorphology of Isolated Rabbit Heart Muscle Mitochondria and the Oxidation of Extramitochondrial Reduced Diphosphopyridine Nucleotide
The morphology of rabbit heart muscle mitochondria isolated in several media has been compared by electron microscopy. The internal structure of isolated mitochondria differs from that of in situ mitochondria, with the type and degree of alteration depending on the isolation medium. Examination of the isolated mitochondria after incubation revealed that additional morphological changes occurred...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1972
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)45582-8